五月丁香六月婷婷综合,亚洲欧美日韩台湾色无极,无套中出丰满人妻无码香港,性aa无码专区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1622KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞

KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞

簡要描述:KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-1622
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-21
  • 訪  問  量:1813

產(chǎn)品分類

Product Category

詳細(xì)介紹

KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞

ATCC® Number:CRL-1622™    Price:$338.00
Designations:KLE

Depositors:GR Richardson

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:


Source:Organ: uterus
Tissue: endometrium
Disease: adenocarcinoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
KLE細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞

Tumorigenic:Yes

Antigen Expression:blood type O; Rh+

DNA Profile (STR):Amelogenin: X

CSF1PO: 13,14

D13S317: 12

D16S539: 11,12

D5S818: 9,12

D7S820: 11,12

THO1: 6,7

TPOX: 8,11

vWA: 16



Age:64 years adult

Gender:female

Ethnicity:Caucasian

Comments:Electron microscopy of tumors formed in nude mice shows microvilli and junctional complexes, and nucleolar channel systems are present that are similar to those seen in normal endometrium under progestational stimulation. The tumors do not form glands.

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:                    
  1. Remove and discard cult   ure medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week


Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
人妻中文在线| 在线观看亚洲av每日更新| 99久久亚洲日本系列| 日韩国产欧美三级片| 日韩欧美一区不卡| 精品无码另类| 国产精品久久久久久无码五月| 一区二区三区四区视频色| 99色热视频精品| 性爱视频二区三区| 日日夜夜噜啊噜| 国产av骚屄| 啪啪网扯| 西西人体大胆免费视频| 国产一区二区三日韩无码| 成人精品视频一区二区三区| 国产麻豆HDvideo无码| 四虎影音最新| 久久久久久久久9| 一本大道东京在线无码| 高清色惰WWW日本午夜色视频| 国产熟女首页| 欧美日韩中字国产| 久久R久久免费视频| 91亚洲精品中文| 欧美激情国产日韩精品一区18| 一级a一级a爱片免费视频| 国产成人黄色精品| 久久人妻午夜| 欧美一区二区在线网站| 乱色熟女综合一区二区三区| 日 韩 色 导航| 《一本久久a久久精品亚洲| 国产国拍亚洲精品五月天婷婷| 精品毛片免費資源站| 亚洲国产精品久久网午夜| 天堂a亚洲v在线a| 狠狠人妻久久久久久综合伊人九色| 九九九久久性爱视频| 嫩草日本| 国产拍揄自揄免费观看|