五月丁香六月婷婷综合,亚洲欧美日韩台湾色无极,无套中出丰满人妻无码香港,性aa无码专区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-5974 SNU-16 人胃癌細(xì)胞 腹水

CRL-5974 SNU-16 人胃癌細(xì)胞 腹水

簡(jiǎn)要描述:CRL-5974 SNU-16 人胃癌細(xì)胞 腹水,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:1961

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-5974 SNU-16 人胃癌細(xì)胞 腹水

ATCC® Number: CRL-5974™ Price: $425.00

Designations: SNU-16

Depositors: J Park

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: stomach

Disease: gastric carcinoma

Derived from metastatic site: ascites

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-5974 SNU-16 人胃癌細(xì)胞 腹水

Isolation: Isolation date: 1987

Receptors: vasoactive intestinal peptide (VIP), expressed [23078]

Oncogene: myc +; erb B2 +

Antigen Expression: Blood Type A; Rh +

The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 8,12

D16S539: 11,13

D5S818: 10,13

D7S820: 12

THO1: 6,9

TPOX: 11

vWA: 16

Cytogenetic Analysis: This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.

Age: 33 years

Gender: female

Ethnicity: Asian

Comments: SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 27 hrs

References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products




ATCC® Number: CRL-5974™ Price: $425.00

Designations: SNU-16

Depositors: J Park

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens (human)

Morphology: epithelial

CRL-5974 SNU-16 人胃癌細(xì)胞 腹水

Source: Organ: stomach

Disease: gastric carcinoma

Derived from metastatic site: ascites

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1987

Receptors: vasoactive intestinal peptide (VIP), expressed [23078]

Oncogene: myc +; erb B2 +

Antigen Expression: Blood Type A; Rh +

The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 8,12

D16S539: 11,13

D5S818: 10,13

D7S820: 12

THO1: 6,9

TPOX: 11

vWA: 16

Cytogenetic Analysis: This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.

Age: 33 years

Gender: female

Ethnicity: Asian

Comments: SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 27 hrs

References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲一区二区三区同男男| 久久国产精品影视| 美女高潮无遮挡高清毛片免费 | 久久特大黄片| 精品久久亚洲中文字幕| 丁香五香天堂网| 久9这里只有精品| 国产午夜无码福利在线看网站 | 四虎4545Www国产精品| 麻豆久久午夜| 国产wwwxx在线免费观看| 无码中文字幕精品推荐| 2020不卡国产精品视频| 成年日韩狼友视频在线观看| 日本91精品在线观看| 青草草A V| 少妇人妻内射| 思思超碰| 国产精品特黄特色三级视频| 中文字幕日韩在线女同| 色欧美成人精品a∨| 91精品久久久久无码午夜福利| 草草影院网址在线观看| 久久国产精品免费中文| 老美BBw搡BBBB搡搡搡搡| 国产操逼视频一区二区| 最新AⅤ| 国产免费无码在线看| 女人被爽到高潮视频免费国产 | 亚洲中文久久精品无码站| 色呦呦呦呦呦呦呦| 人妻激情综合| 欧美熟女黑人| 欧美精品777老熟妇2| 无码人妻白浆| 小荡货好紧好爽奶头大视频| 窝窝社区色| www.亚洲日韩| 国产在线观看无码不卡| 自慰在线一区二区三区| 精品国产综合区久久久久久无码 |