五月丁香六月婷婷综合,亚洲欧美日韩台湾色无极,无套中出丰满人妻无码香港,性aa无码专区

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2119HPAC 人胰腺癌細(xì)胞

HPAC 人胰腺癌細(xì)胞

簡(jiǎn)要描述:CRL-2119 HPAC 人胰腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2119
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:1813

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2119 HPAC 人胰腺癌細(xì)胞

ATCC® Number: CRL-2119™ Price: $323.00

Designations: HPAC

Depositors: WR Gower

Biosaum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreasfety Level: 1

Shipped: frozen

Medium & Ser

Disease: adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2119 HPAC 人胰腺癌細(xì)胞

Isolation: Isolation date: 1985

Receptors: epidermal growth factor (EGF), expressed

glucocorticoid, expressed

epidermal growth factor (EGF); glucocorticoid

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 13

D13S317: 11

D16S539: 9,10

D5S818: 12

D7S820: 10,12

THO1: 9.3

TPOX: 10,11

vWA: 15,17

Cytogenetic Analysis: modal number = 61

Age: 64 years

Gender: female

Ethnicity: Caucasian

Comments: HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin.

HPAC proliferation is stimulated by insulin, insulin-like growth factor I (IGF-I), epidermal growth factor (EGF), and transforming growth factor alpha (TGF alpha).

Cell growth is suppressed by dexamethasone and other glucocorticoids.

HPAC cells are positive for keratin and negative for vimentin and chromogranin A.

Immunohistochemical staining revealed that HPAC cells express the pancreatic ductal epithelium marker DU-PAN-2 as well as antigens recognized by the monoclonal antibodies HMFG1 and AUA1.

Crude HPAC cell exacts contained significant concentrations of tumor-associated antigens CEA, CA 125, and CA 19-9.

In culture, HPAC cells form monolayers of morphologically heterogenous polar epithelial cells.

HPAC is the first reported human pancreatic adenocarcinoma cell line to express a functional glucocorticoid receptor.

Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 0.002 mg/ml insulin, 0.005 mg/ml transferrin, 40 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor and 5% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 41 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 22987: Gower WR Jr., et al. HPAC, a new human glucocorticoid-sensitive pancreatic ductal adenocarcinoma cell line. In Vitro Cell. Dev. Biol. 30A: 151-161, 1994.



CRL-2119


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
日本中文字幕在线视频站| 亚洲精品啪| 美日韩无码专区| 77777日本欧美在线| 极品福利久久久| 骚笔爱人妻| 亚洲精品无码专区在线观看| 亚洲无码在线中文| 亚洲综合一区二区在线观看的| 999国产精品亚洲| 日本三级片99| 精品无码一区二区爱欲| 欧美亚洲少妇日韩| 国产自产拍精品视频免费看| 性色AV无码久久久久久免| 国产深喉口爆吞精在线视| 中文字幕欧美aⅴ字幕| 午夜污在线| 欧美日韩中文字幕小说| 欧美日韩一区二区三区人妻| xxxx日日摸夜夜添夜夜添视频| 中国毛茸茸勉费视频| 久久漂亮人妻洗澡被公强| 涩涩视频网| 国产视频5| 色鬼综合网| 亚洲精品人中文字幕高潮| 无码专区—va亚洲v专区| 欧美一区二区成人熟女| www69成人| 国产精品禁18久久久夂久| 男人扒开女人下部添高潮的视频| 亚洲老司机中文字幕| 丁香五月婷婷激情伊人| 中国女人内射在线视频| 无码中文人妻字偷2020| 中文字幕在线无码视频| 国产最新一区二区三区天堂| 亚洲av综合无码一区| 人妻丰满熟妇av无码区hd| 中文字幕激情另类|